The DAFNE-Light laboratory offers diagnostic analyzes and the development of innovative investigation technologies for the non-destructive or micro-destructive characterization of the materials constituting the cultural heritage (pigments, binders, consolidants, dyes) and their degradation products, using spectroscopic techniques ( FT-IR, Raman, XRF, absorption X) and imaging (FT-IR, UV, SWIR, multispectral VIS-NIR, SEM). In addition to laboratory instrumentation with conventional sources, the presence of a synchrotron light source from infrared to X-rays allows precise analysis and images on micrometric samples. Along with laboratory instrumentation, portable instrumentation is available to facilitate in-situ measurements and non-destructive investigation methodologies.


Molecular analyses:

  • FT-IR Bruker Vertex-70V (Far/Mid/Near Infrared) interferometer
  • Tensor (Mid Infrared) interferometer equipped with autosampler module HTS-XT
  •  FT-IR Bruker Hyperion 3000 infrared microscope with FPA (Focal Plane Array) 64×64 pixel imaging detector
  • Micro and macro ATR (Attenuated Total Reflection)
  • Micro Raman Jasco NRS-5100 (785nm, 532nm)
  • Fluorescence microscopy (355 nm)


Elemental analyses:

  • SEM (Scanning Electron Microscope) with microanalysis EDX


Sample preparation:

  • Optical microscopy
  • Cutter
  • Ventilated room
  • Preparation of cross-sections embedded in resin


In situ analyses:

  • FT-IR spectroscopy: FT-IR, Bruker Alpha-R (350-7000 cm-1)
  • i-Raman Plus B&W Tek (laser 785nm)
  • Led-induced fluorescence spectroscopy (280 nm, 355 nm, 450 nm)
  • UV fluorescence imaging (Camera NIR converted Nikon D750, UV lamp at 365nm filtered 3000mW)
  •  Multispcetral Imaging (Camera NIR converted Nikon D750, 400-1000 nm con filtri a banda stretta VIS-IR)
  • SWIR reflectance camera (Hamamatsu InGaAs Camera C12741-03, 640×512 pixel) 900-1700 nm, halogen lamp 250W
  • NIR reflectance (Fotocamera NIR converted Nikon D750,  750-1000 nm filter), halogen lamp 250W
  • Macro and micro photography: Nikon D750 and DinoLite portable microscope


The laboratory is part of the R6 Network – Chemical-physical sciences and technologies belonging to the Center of Excellence of the Technological District of Cultural Heritage and Activities (DTC) of the Lazio Region.